Merck MISSION Lenti microRNA Inhibitor, Mouse

MISSION® Lenti microRNA Inhibitor, Mouse

mmu-miR-1231-3p

Design Type: Tough Decoy (TuD)

제품 정보

제품 설명

Individual lenti microRNA inhibitors are designed using a proprietary algorithm based on the work of Haraguchi, T. et al. in collaboration with Dr. Hideo Iba (University of Tokyo).
This algorithm utilizes the Tough Decoy (TuD) design to achieve potent inhibition of target miRNA.

miRNAs regulate gene expression through various mechanisms, including translational repression, mRNA cleavage, and deadenylation.
The lentiviral microRNA inhibitors are cloned into the TRC2-pLKO-puro vector.
Co-transfection of this vector with compatible packaging plasmids produces viral particles suitable for transduction into mammalian cells.

Additionally, the Woodchuck Hepatitis Post-Transcriptional Regulatory Element (WPRE) enhances transgene expression, and the vector includes a puromycin resistance gene for cell selection.

주요 특징

• 강력하고 지속적인 miRNA 억제 가능
• 렌티바이러스 전달 형식으로 다양한 세포 유형에 효율적 도입
• 반복적인 트랜스펙션 없이 장기 억제 효과 유지
• 퓨로마이신 저항성을 통한 안정적 세포 선택 가능