Merck MISSION Lenti microRNA Inhibitor, Mouse

MISSION® Lenti microRNA Inhibitor, Mouse

mmu-miR-5130

Design: Tough Decoy (TuD)

제품 정보

제품 설명

Individual lenti microRNA inhibitors are designed using a proprietary algorithm based on the work of Haraguchi, T. et al. in collaboration with Dr. Hideo Iba (University of Tokyo).
This algorithm utilizes the Tough Decoy (TuD) design, enabling potent inhibition of specific miRNAs.

miRNAs regulate gene expression through translational repression, mRNA cleavage, and deadenylation.
These lentiviral microRNA inhibitors are cloned into the TRC2-pLKO-puro vector, which, when co-transfected with compatible packaging plasmids, produces viral particles suitable for transducing mammalian cells.

The vector includes:

• WPRE (Woodchuck Hepatitis Post-Transcriptional Regulatory Element) for enhanced expression of delivered transgenes
• Puromycin resistance gene for cell selection

주요 특징

• 강력한 목표 miRNA 억제 가능
• 렌티바이러스 전달 형식으로 다양한 세포 유형에 효율적 전달
• 반복 형질감염 없이 장기 억제 가능