Merck MISSION Lenti microRNA Inhibitor, Mouse

MISSION® Lenti microRNA Inhibitor, Mouse

mmu-miR-412-3p

Tough Decoy (TuD) 설계 기반

제품 정보

제품 설명

Individual lenti microRNA inhibitors are designed using a proprietary algorithm developed in collaboration with Dr. Hideo Iba (University of Tokyo), based on the work of Haraguchi et al. This algorithm employs the Tough Decoy (TuD) design to effectively inhibit specific miRNA activity.

miRNA regulate gene expression through mechanisms such as translational repression, mRNA cleavage, and deadenylation. The lentiviral microRNA inhibitors are cloned into the TRC2-pLKO-puro vector, which enables co-transfection with compatible packaging plasmids to produce viral particles for transduction into mammalian cells.

The vector includes:

• Woodchuck Hepatitis Post-Transcriptional Regulatory Element 2 (WPRE) for enhanced transgene expression
• Puromycin resistance gene for cell selection

주요 특징

• 강력한 miRNA 억제 효과
• 렌티바이러스 전달 형식으로 다양한 세포 유형에 효율적 적용
• 반복적인 트랜스펙션 없이 장기적 억제 가능