Merck MISSION Lenti microRNA Inhibitor, Mouse

MISSION® Lenti microRNA Inhibitor, Mouse

mmu-miR-3474

Tough Decoy (TuD)

제품 개요

Individual lenti microRNA inhibitors are designed using a proprietary algorithm based on the work of Haraguchi, T. et al., in collaboration with Dr. Hideo Iba (University of Tokyo).
This algorithm utilizes the Tough Decoy (TuD) design.
miRNA are known to regulate gene expression through translational repression, mRNA cleavage, and deadenylation.

The lentiviral microRNA inhibitors are cloned into the TRC2-pLKO-puro vector.
Co-transfection of this vector with compatible packaging plasmids produces viral particles used to transduce mammalian cells.
The Woodchuck Hepatitis Post-Transcriptional Regulatory Element2 (WPRE) enhances transgene expression, and the vector includes a puromycin resistance gene for cell selection.

주요 특징

• Potent inhibition of target miRNA
• Lentiviral delivery for efficient transduction across diverse cell types
• Enables long-term inhibition without repeat transfection

제품 스펙