Merck MISSION Lenti microRNA Inhibitor, Mouse

MISSION® Lenti microRNA Inhibitor, Mouse

대상 microRNA

• mmu-miR-223-5p

형태 및 특징

• 형태(Form): Liquid
• 농도(Concentration): ≥1×10⁶ VP/ml (via p24 assay)
• 배송 상태(Shipping Condition): Dry ice
• 저장 온도(Storage Temperature): −70°C

서열 정보

품질 등급

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기술 설명

Individual lenti microRNA inhibitors are designed using a proprietary algorithm developed in collaboration with Dr. Hideo Iba (University of Tokyo), based on the work of Haraguchi et al. This algorithm employs the Tough Decoy (TuD) design.

miRNAs regulate gene expression through various mechanisms such as translational repression, mRNA cleavage, and deadenylation. The lentiviral microRNA inhibitors are cloned into the TRC2-pLKO-puro vector. Co-transfection with compatible packaging plasmids produces viral particles for mammalian cell transduction.

The vector includes the Woodchuck Hepatitis Post-Transcriptional Regulatory Element 2 (WPRE) for enhanced transgene expression, and a puromycin resistance gene for selection of transduced cells.

주요 특징

• 강력한 목표 miRNA 억제 가능
• 렌티바이러스 전달 형식으로 다양한 세포에 효율적 도입
• 반복적인 트랜스펙션 없이 장기적 억제 유지 가능

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