Merck MISSION Lenti microRNA Inhibitor, Mouse

MISSION® Lenti microRNA Inhibitor, Mouse

mmu-miR-379-3p

형태: Tough Decoy (TuD)

제품 스펙

제품 설명

Individual lenti microRNA inhibitors are designed using a proprietary algorithm based on the work of Haraguchi, T. et al., in collaboration with Dr. Hideo Iba (University of Tokyo).
This algorithm utilizes the Tough Decoy (TuD) design for effective inhibition of target miRNA.

miRNA regulate gene expression through mechanisms such as translational repression, mRNA cleavage, and deadenylation.
The lentiviral microRNA inhibitors are cloned into the TRC2-pLKO-puro vector. Co-transfection with compatible packaging plasmids produces viral particles suitable for mammalian cell transduction.

Additionally, the Woodchuck Hepatitis Post-Transcriptional Regulatory Element2 (WPRE) enhances transgene expression, and the vector includes a puromycin resistance gene for cell selection.

주요 특징

• 원하는 miRNA의 강력한 억제 가능
• 렌티바이러스 전달 형식으로 다양한 세포주에 효율적 적용
• 반복적인 트랜스펙션 없이 장기적인 miRNA 억제 가능
• 퓨로마이신 내성 유전자를 통한 안정적 세포 선택