Merck MISSION pLKO.1-puro TurboGFP shRNA Control Transduction Particles, High Titer

MISSION® pLKO.1-puro TurboGFP™ shRNA Control Transduction Particles, High Titer

High Titer, shRNA sequence targeting tGFP

MISSION TurboGFP Control Transduction Particles

Quality Level

200

제품 라인

MISSION®

농도

≥1x109 VP/ml (via p24 assay)

technique(s)

capture ELISA: 109 TU/mL using p24

배송 상태

dry ice

저장 온도

−70°C

The MISSION® TurboGFP™ shRNA Control Transduction Particles contain an shRNA sequence targeting TurboGFP. The TurboGFP shRNA Control Particles are useful as a positive knockdown control in experiments using the MISSION TurboGFP positive control vector or in cell lines expressing TurboGFP. TurboGFP is an improved variant of the green fluorescent protein copGFP cloned from copepoda Pontellina plumata.

The TurboGFP shRNA control transduction particles are produced from the sequence-verified lentiviral plasmid, pLKO.1-puro-TurboGFP shRNA (SHC004). Self-inactivating replication incompetent viral particles are produced in packaging cells (HEK293T) by co-transfection with compatible packaging plasmids. In addition, the Control Transduction Particles are pseudotyped with an envelope G glycoprotein from Vesicular Stomatitis Virus (VSV-G), allowing transduction of a wide variety of mammalian cells. 200 μl of 109 TU/ml (via p24 titering assay) lentiviral particles are provided as frozen stock.

This high titer offering is recommended for customers: who need to utilize smaller volumes of virus, who have very difficult to transduce cell lines, or who need to transduce large numbers of cells.