Merck MISSION pLKO.1-puro Non-Target shRNA Control Plasmid DNA

MISSION® pLKO.1-puro Non-Target shRNA Control Plasmid DNA

Targets no known genes from any species

negative shRNA control, shRNA control, MISSION Control Vectors, negative control, non-target shRNA, non-target control, non-target shRNA control

Quality Level

200

제품 라인

MISSION®

농도

500 ng/μL in TE buffer; DNA (10μg of plasmid DNA)

배송 상태

dry ice

저장 온도

−20°C

The MISSION pLKO.1-puro Non-Target shRNA Control Plasmid DNA is a lentivirus plasmid vector. The vector contains an shRNA insert that does not target any known genes from any species, making it useful as a negative control in experiments using the MISSION shRNA library clones. This allows one to examine the effect of transfection of a short-hairpin on gene expression and interpret the knockdown effect seen with shRNA clones. Ampicillin and puromycin antibiotic resistance genes provide selection in bacterial or mammalian cells respectively. In addition, self-inactivating replication incompetent viral particles can be produced in packaging cells (HEK293T) by co-transfection with compatible packaging plasmids. The Non-Target shRNA Control Plasmid DNA is provided as 10 μg of plasmid DNA in Tris-EDTA (TE) buffer at a concentration of 500 ng/μl.