Merck ChIPAb+ Dimethyl-Histone H4 (Arg3) Symmetric - ChIP Validated Antibody and Primer Set

ChIPAb+ Dimethyl-Histone H4 (Arg3) Symmetric - ChIP Validated Antibody and Primer Set

from rabbit, purified by affinity chromatography

Histone H4

생물학적 소스

rabbit

Quality Level

100

클론

polyclonal

정제법

affinity chromatography

species reactivity

mouse, human, rat

manufacturer/tradename

ChIPAb+
Upstate®

technique(s)

ChIP: suitable
dot blot: suitable
western blot: suitable

NCBI 수납 번호

NP_001029249

UniProt 수납 번호

P62805

배송 상태

dry ice

Histones are highly conserved proteins that serve as the structural scaffold for the organization of nuclear DNA into chromatin. The four core histones, H2A, H2B, H3, and H4, assemble into an octamer (2 molecules of each). Histones are modified post-translationally by the actions of enzymes in both the nucleus and cytoplasm. These modifications, which include acetylation, phosphorylation, methylation, and ubiquitin-ation, occur predominantly on the N-terminal and C-terminal tails that extend beyond the nucleosome core particle. Arginine modifications of histones proteins are mediated by two classes of protein arginine methyltransferases (PRMTs), class I and II. Class I PRMTs place two methyl groups on the one nitrogen atom of the arginine guanidino group, resulting in an asymmetric modification. In contrast class II PRMTs place two methyl groups on two nitrogen atoms of the guanidino group producing a symmetric dimethylation. Although there are exceptions, asymmetric modifications are typically associated with transcription activation while symmetric modifications are most commonly associated with transcriptional repression.