Merck PSF-CMV-NH2-CMYC-EKT-NCOI - N-TERMINAL C-MYC PLASMID

PSF-CMV-NH2-CMYC-EKT-NCOI - N-TERMINAL C-MYC PLASMID

plasmid vector for molecular cloning

expression vector, molecular cloning vector, vector, snapfast vector, plasmid vector, cloning vector, plasmid

태그

c-Myc tagged

form

buffered aqueous solution

분자량

size 4268 bp

박테리아 선정

kanamycin

복제개시점

pUC (500 copies)

펩타이드 절단

no cleavage

펩타이드 태그 위치

N-terminal

프로모터

Promoter name: CMV
Promoter activity: constitutive
Promoter type: mammalian

리포터 유전자

none

배송 상태

ambient

저장 온도

−20°C

This plasmid adds a CMyc epitope tag to the N-terminus of a protein that is encoded within the multiple cloning site. This tag allows the detection and purification of a tagged protein using antibodies raised against the CMyc epitope. The CMyc tag coding sequence is EQKLISEEDL. There is an enterokinase cleavage site (DDDDK) immediately downstream of the CMyc tag that can be used to remove it from a purified protein if required. It cleaves after the lysine residue.

Promoter Expression Level: This plasmid contains the mammalian CMV promoter to drive gene expression. We have tested all of our mammalian promoters in a range of cell types and CMV is consistently the strongest in those we have studied. However there are many reports of the CMV promoter demonstrating silencing by methylation in long-term culture.