Merck PSF-TEF1-SNAPFUSION - YEAST SNAPFUSION CLONING PLASMID

PSF-TEF1-SNAPFUSION - YEAST SNAPFUSION CLONING PLASMID

plasmid vector for molecular cloning

expression vector, molecular cloning vector, vector, snapfast vector, plasmid vector, cloning vector, plasmid

form

buffered aqueous solution

분자량

size 6728 bp

박테리아 선정

kanamycin

복제개시점

2Micron
pUC (500 copies)

펩타이드 절단

no cleavage

프로모터

Promoter name: TEF1
Promoter activity: constitutive
Promoter type: yeast

리포터 유전자

none

배송 상태

ambient

저장 온도

−20°C

효모 선정

uracil

This vector is a standard Saccharomyces cerevisiae yeast expression plasmid with the exception that it has been modified to allow the direct cloning of gene fragments that have been designed to fit with any of our peptide tag plasmid range. This can be useful if you have created a peptide fusion but you would also like to create a non-tagged gene variant. The multiple cloning site contains two BsgI and BseRI sites that produce overhangs that are compatible with gene designed using our SnapFusion primer designer (see the cloning tab for this product). The promoter in this plasmid (TEF1) is a strong constitutive yeast promoter.

Promoter Expression Level: This plasmid contains the yeast translation elongation factor 1 promoter. It is the strongest promoter that we provide for expression in Saccharomyces cerevisiae.