Merck PSF-CMV-PGK-KRYFP - YFP MAMMALIAN PGK PROMOTER PLASMID

PSF-CMV-PGK-KRYFP - YFP MAMMALIAN PGK PROMOTER PLASMID

plasmid vector for molecular cloning

expression vector, molecular cloning vector, vector, snapfast vector, plasmid vector, cloning vector, plasmid

form

buffered aqueous solution

분자량

size 5447 bp

박테리아 선정

kanamycin

복제개시점

pUC (500 copies)

펩타이드 절단

no cleavage

프로모터

Promoter name: CMV
Promoter activity: constitutive
Promoter type: mammalian

리포터 유전자

YFP

배송 상태

ambient

저장 온도

−20°C

This plasmid vector contains the CMV promoter upstream of the MCS and phosphoglycerate kinase (PGK) promoter driving expression of Kringle YFP (Yellow Fluorescence Protein)

Promoter Expression Level: This plasmid contains the mammalian CMV promoter to drive gene expression. We have tested all of our mammalian promoters in a range of cell types and CMV is consistently the strongest in those we have studied. However there are many reports of the CMV promoter demonstrating silencing by methylation in long-term culture. The murine phosphoglycerate kinase (PGK) promoter driving reporter gene expression allows for long term stable expression in vitro and in vivo in most types.