Merck PSF-MINCMV-NEO/G418 - G418 SELECTION MINIMAL CMV VECTOR

PSF-MINCMV-NEO/G418 - G418 SELECTION MINIMAL CMV VECTOR

plasmid vector for molecular cloning

expression vector, molecular cloning vector, vector, snapfast vector, plasmid vector, cloning vector, plasmid

form

buffered aqueous solution

분자량

size 4525 bp

박테리아 선정

kanamycin

포유류 세포 선정

neomycin / G418

복제개시점

pUC (500 copies)

펩타이드 절단

no cleavage

프로모터

Promoter name: CMV
Promoter activity: minimal promoter
Promoter type: mammalian

리포터 유전자

none

배송 상태

ambient

저장 온도

−20°C

Here the neomycin/G418 resistance gene is under transcriptional control of the minimal CMV promoter giving basal levels of expression in most mammalian cells. An upstream MCS is positioned to allow inclusion of additional regulatory elements upstream of the minimal promoter allowing high levels of resistance to be conditional upon binding of specific transcription factors or on the presence of externally-applied drugs such as doxycycline. In this way the plasmid allows production of a range of sophisticated constructs providing G418/neomycin resistance only under specified conditions.

Promoter Expression Level: This plasmid contains the minimal CMV immediate early promoter with a multiple cloning site immediately upstream that allows transcription factor binding sites to be inserted. This allows tissue specific or physiologically responsive promoters to be created.