Merck PSF-CMV-FMDV-FRCFP - FMDV IRES CFP REPORTER PLASMID

PSF-CMV-FMDV-FRCFP - FMDV IRES CFP REPORTER PLASMID

plasmid vector for molecular cloning

expression vector, molecular cloning vector, vector, snapfast vector, plasmid vector, cloning vector, plasmid

form

buffered aqueous solution

분자량

size 5427 bp

박테리아 선정

kanamycin

복제개시점

pUC (500 copies)

펩타이드 절단

no cleavage

프로모터

Promoter name: CMV
Promoter activity: constitutive
Promoter type: mammalian

리포터 유전자

CFP

배송 상태

ambient

저장 온도

−20°C

This vector contains a CMV promoter that allows the expression of a transgene in mammalian cells. The plasmid also includes the internal ribosome entry site (IRES) from Foot and Mouth Disease Virus (FMDV) which allows two proteins to be translated from the same mRNA molecule. In this vector there is a cyan fluorescent protein (CFP) reporter gene after the IRES. FMDV IRES expression is often signficantly lower (typically 20-30 fold lower by total protein weight) than the gene upstream of the IRES. The reporter in this plasmid is called Frosty CFP and was developed by DNA2. 0.

Promoter Expression Level: Promoter and IRES Expression Level: This plasmid contains the mammalian CMV promoter to drive gene expression. We have tested all of our mammalian promoters in a range of cell types and CMV is consistently the strongest in those we have studied. However there are many reports of the CMV promoter demonstrating silencing by methylation in long-term culture. The IRES that is used to drive CFP expression from the CMV promoter is considerably weaker than the CMV promoter and typically yields >20 fold lower protein levels.