Merck pSF-CMV-NEO-NH2-3XFLAG-COOH-Cmyc

pSF-CMV-NEO-NH2-3XFLAG-COOH-Cmyc

plasmid vector for molecular cloning

expression vector, molecular cloning vector, vector, snapfast vector, plasmid vector, cloning vector, plasmid

재조합

expressed in human cells

태그

3X FLAG tagged
c-Myc tagged

form

buffered aqueous solution

분자량

size 5931 bp

박테리아 선정

ampicillin

복제개시점

pUC

펩타이드 절단

EKT

펩타이드 태그 위치

C-terminal
N-terminal

프로모터

Promoter name: CMV
Promoter activity: constitutive
Promoter type: mammalian

배송 상태

ambient

저장 온도

−20°C

The pSF-CMV-NEO-NH2-3XFLAG-COOH-Cmyc expression vector is a 5.9 kb derivative of pSF-CMV-Amp for transient or stable expression of intracellular dual-tagged N-terminal 3XFLAG and C-terminal c-myc fusion proteins in mammalian cells. This vector encodes three FLAG epitopes in tandem (DYKDHDG-DYKDHDI-DYKDDDDK), which results in increased sensitivity when using the anti-FLAG M2 antibody (catalogue number F3165). The third epitope includes the enterokinase recognition sequence, allowing cleavage of the 3XFLAG peptide from the purified fusion protein. A c-myc epitope tag (EQKLISEEDL) is present downstream of the multiple cloning site. pSF-CMV-NEO-NH2-3XFLAG-COOH-Cmyc is a shuttle vector, containing both E. coli and SV40 origins of replication, for propagation in bacterial and mammalian cells. Efficiency of replication and genomic integration is optimal when using an SV40 T antigen expressing mammalian host. The promoter regulatory region of the human cytomegalovirus drives transcription of FLAG and c-myc fusion constructs. The aminoglycoside phosphotransferase II gene (neo-R) confers resistance to aminoglycosides such as G418, allowing for selection of stable transfectants.