Merck PSF-TEFI-TPI1-FLUC-URA3 - FIREFLY LUCIFERASE YEAST VECTOR

PSF-TEFI-TPI1-FLUC-URA3 - FIREFLY LUCIFERASE YEAST VECTOR

plasmid vector for molecular cloning

expression vector, molecular cloning vector, vector, snapfast vector, plasmid vector, cloning vector, plasmid

form

buffered aqueous solution

분자량

size 9226 bp

박테리아 선정

kanamycin

복제개시점

2Micron
pUC (500 copies)

펩타이드 절단

no cleavage

프로모터

Promoter name: TEF1
Promoter activity: constitutive
Promoter type: yeast

리포터 유전자

firefly luciferase

배송 상태

ambient

저장 온도

−20°C

효모 선정

uracil

A dual transgene Saccharomyces cerevisiae yeast expression plasmid that allows the co-expression of luciferase alongside a gene of interest. The vector also contains a gene that is essential for uracil synthesis allowing the plasmids to be maintained in cells that are defective for this gene when they are grown in media that is missing uracil.

Promoter Expression Level: This plasmid contains the yeast translation elongation factor 1 promoter. It is the strongest promoter that we provide for expression in Saccharomyces cerevisiae. It also contains the strong yeast constitutive triosephosphate isomerase (TPI1) gene promoter to drive the expression of the reporter gene. The TPI promoter demonstrates similar levels of expression to the translation elongation factor 1 promoter (TEF-1).