Merck PSF-TEFI-HIS3 - HISTIDINE YEAST SELECTION PLASMID

PSF-TEFI-HIS3 - HISTIDINE YEAST SELECTION PLASMID

plasmid vector for molecular cloning

expression vector, molecular cloning vector, vector, snapfast vector, plasmid vector, cloning vector, plasmid

form

buffered aqueous solution

분자량

size 6800 bp

박테리아 선정

kanamycin

복제개시점

2Micron
pUC (500 copies)

펩타이드 절단

no cleavage

프로모터

Promoter name: TEF1
Promoter activity: constitutive
Promoter type: yeast

리포터 유전자

none

배송 상태

ambient

저장 온도

−20°C

The purpose of this expression vector is the expression of genes in Saccharomyces cerevisiae using selective removal of histidine from the media for cell selection. The plasmid contains a gene that is an essential part of the histidine synthesis pathway. By transforming the plasmid into yeast cells that are deficient in this gene and growing the cells on media that does not contain histidine cells can be selected that contain the plasmid. This is one of a range of metabolic selection genes that are available for yeast. Others include tryptophan leucine and uracil. We also provide plasmids using more standard selection methods such as puromycin and hygromycin.

Promoter Expression Level: This snapfast vector contains the yeast translation elongation factor 1 promoter. It is the strongest promoter that we provide for expression in Saccharomyces cerevisiae.