Merck PSF-CMV-HYGRO - CMV DRIVEN HYGROMYCIN RESISTANT VECTOR

PSF-CMV-HYGRO - CMV DRIVEN HYGROMYCIN RESISTANT VECTOR

plasmid vector for molecular cloning

expression vector, molecular cloning vector, vector, snapfast vector, plasmid vector, cloning vector, plasmid

form

buffered aqueous solution

분자량

size 5242 bp

박테리아 선정

kanamycin

포유류 세포 선정

hygromycin

복제개시점

pUC (500 copies)

펩타이드 절단

no cleavage

프로모터

Promoter name: CMV
Promoter activity: constitutive
Promoter type: mammalian

리포터 유전자

none

배송 상태

ambient

저장 온도

−20°C

Here the hygromycin gene is under transcriptional control of the CMV promoter allowing simple selection of transfected mammalian cells using hygromycin.

Promoter Expression Level: This plasmid contains the mammalian CMV promoter to drive gene expression. We have tested all of our mammalian promoters in a range of cell types and CMV is consistently the strongest in those we have studied. However there are many reports of the CMV promoter demonstrating silencing by methylation in long-term culture. For this reason we stock a range of other promoters that are compatible with this plasmid and are available on request.