Merck PSF-TEFI-CEN6/ARS4-URA3 - LOW COPY ORIGIN YEAST PLASMID

PSF-TEFI-CEN6/ARS4-URA3 - LOW COPY ORIGIN YEAST PLASMID

plasmid vector for molecular cloning

expression vector, molecular cloning vector, vector, snapfast vector, plasmid vector, cloning vector, plasmid

form

buffered aqueous solution

분자량

size 5892 bp

박테리아 선정

kanamycin

복제개시점

pUC (500 copies)

펩타이드 절단

no cleavage

프로모터

Promoter name: TEF1
Promoter activity: constitutive
Promoter type: yeast

리포터 유전자

none

배송 상태

ambient

저장 온도

−20°C

효모 선정

uracil

This is a low copy Saccharomyces cerevisiae yeast plasmid that allows for the plasmid to be maintained in cells at an approximate frequency of 1-2 copies per cell. These plasmids are not typically used for protein production because expression is signficantly lower than using the standard 2 micron origin which has a much higher copy number. This plasmid can still be used when large DNA inserts are required and low levels of expression of genes are needed. The plasmid contains the URA3 selection cassette which allows the plasmid to be maintained in cells that are defective for this gene. Cells carrying the plasmid can then be grown in media that is deficient in uracil.

Promoter Expression Level: This expression vector contains the yeast translation elongation factor 1 promoter. It is the strongest promoter that we provide for expression in Saccharomyces cerevisiae.