Merck PSF-CMV-BLAST - CMV PROMOTER BLASTICIDIN RESISTANT PLASMID

PSF-CMV-BLAST - CMV PROMOTER BLASTICIDIN RESISTANT PLASMID

plasmid vector for molecular cloning

expression vector, molecular cloning vector, vector, snapfast vector, plasmid vector, cloning vector, plasmid

form

buffered aqueous solution

분자량

size 4639 bp

박테리아 선정

kanamycin

포유류 세포 선정

blasticidin

복제개시점

pUC (500 copies)

펩타이드 절단

no cleavage

프로모터

Promoter name: CMV
Promoter activity: constitutive
Promoter type: mammalian

리포터 유전자

none

배송 상태

ambient

저장 온도

−20°C

Here the CMV promoter regulates expression of blasticidin resistance allowing simple selection of transfected mammalian cells by blasticidin selection.

Promoter Expression Level: PSF-CMV-BLAST - CMV promoter blasticidin resistant plasmid contains the mammalian CMV promoter to drive gene expression. We have tested all of our mammalian promoters in a range of cell types and CMV is consistently the strongest in those we have studied. However there are many reports of the CMV promoter demonstrating silencing by methylation in long-term culture.

PSF-CMV-BLAST - CMV promoter blasticidin resistant plasmid is a blasticidin resistant plasmid where expression is controlled by the CMV promoter. It allows the production of mammalian cell lines carrying the plasmid.