Merck PSF-CMV-HUKAPPA LC - HUMAN KAPPA LIGHT CHAIN ANTIBODY VECTOR

PSF-CMV-HUKAPPA LC - HUMAN KAPPA LIGHT CHAIN ANTIBODY VECTOR

plasmid vector for molecular cloning

expression vector, molecular cloning vector, vector, snapfast vector, plasmid vector, cloning vector, plasmid

form

buffered aqueous solution

분자량

size 4579 bp

박테리아 선정

kanamycin

복제개시점

pUC (500 copies)

펩타이드 절단

no cleavage

프로모터

Promoter name: CMV
Promoter activity: constitutive
Promoter type: mammalian

리포터 유전자

none

배송 상태

ambient

저장 온도

−20°C

This is an antibody expression plasmid designed for the generation of full length human kappa light chain expression cassettes. The vector has been designed to contain a non-palindomic resitriction site called BseRI upstream from the light chain coding region. When this enzyme is used it produces an overhang from the first two two nucleotides in the first codon of the constant region. This allows seamless fusion to be made between variable regions carrying the same overhang. This can be achieved by creating a variable region PCR fragment that contains a BserRI site at the end in the opposing orientation.

Promoter Expression Level: This plasmid contains the mammalian CMV promoter to drive gene expression. We have tested all of our mammalian promoters in a range of cell types and CMV is consistently the strongest in those we have studied. However there are many reports of the CMV promoter demonstrating silencing by methylation in long-term culture.