Merck PSF-EF1-PURO - ELONGATION FACTOR 1 ALPHA PROMOTER PUROMYCIN PLASMID

PSF-EF1-PURO - ELONGATION FACTOR 1 ALPHA PROMOTER PUROMYCIN PLASMID

plasmid vector for molecular cloning

expression vector, molecular cloning vector, vector, snapfast vector, plasmid vector, cloning vector, plasmid

form

buffered aqueous solution

분자량

size 5437 bp

박테리아 선정

kanamycin

포유류 세포 선정

puromycin

복제개시점

pUC (500 copies)

펩타이드 절단

no cleavage

프로모터

Promoter name: EF1-alpha
Promoter activity: constitutive
Promoter type: mammalian

리포터 유전자

none

배송 상태

ambient

저장 온도

−20°C

Here the puromycin resistance gene is under transcription regulation of the human Elongation Factor 1 alpha promoter giving long term expression that can be useful for selection of transfected mammalian cells to make stable cell lines

Promoter Expression Level: Molecular cloning vector contains the human Elongation factor 1 promoter to drive protein expression. The EF1 alpha promoter contains a large intron that helps to maintain expression of the promoter in long term culture. Although it shows signficantly lower expression in most cell types compared to the CMV promoter by transient transfection the promoter can maintain activity for longer in many cell types when making stable cell lines.