Merck Hyaluronidase from bovine testes

Hyaluronidase from bovine testes

Type IV-S, powder, suitable for mouse embryo cell culture, 750-3000 units/mg solid

Hyaluronoglucosaminidase, Hyaluronate 4-glycanohydrolase

Quality Level

200

무균

aseptically processed

유형

Type IV-S

form

powder

specific activity

750-3000 units/mg solid

분자량

~55 kDa (four subunits of 14 kDa each)

구성

Protein, 80-105% biuret

technique(s)

cell culture | embryo: suitable

저장 온도

−20°C

Hyaluronidase is an enzyme derived from bovine testes. It is used to remove the cumulus mass in the isolation of mouse embryos or from newly ovulated eggs, when preparing the eggs for in vitro culture.

Gonadotropins are used to superovulate 20 female mice. The mice are then mated and preimplantation stage embryos are retrieved and counted. The embryos are treated with hyaluronidase until the cumulus mass is dissociated from the embryos. The embryos are cultured, viewed daily and progress of the embryos is recorded.