Merck Tumor Necrosis Factor-β human

Tumor Necrosis Factor-β human

TNF-β, recombinant, expressed in E. coli, powder, suitable for cell culture

TNF-β

Quality Level

200

생물학적 소스

human

재조합

expressed in E. coli

assay

>95% (SDS-PAGE)

form

powder

효능

4-20 pg/mL Activity

품질

endotoxin tested

분자량

protein 18.8 kDa

포장

pkg of 10 μg

저장 조건

avoid repeated freeze/thaw cycles

technique(s)

cell culture | mammalian: suitable

불순물

≤1 EU/μg

UniProt 수납 번호

P01374

저장 온도

−20°C

Gene Information

human ... LTA(4049) , TNFSF13B(10673)

Tumor necrosis factor-β (TNF-β), also known as lymphotoxin-α (LT-α) and cytotoxic factor, is a 25 kDa glycoprotein that is tightly regulated by lymphocytes. TNF-β and TNF-α share a 28% amino acid sequence identity, and as soluble forms, both can exist in solution as homotrimers, activate the same TNF receptors (TNFR1 and TNFR2) and elicit many of the same bioactivities. Unlike TNF-α, TNF-β can also form heterotrimers with one or two molecules of the related membrane-bound lymphotoxin-β (LT-β). Reportedly, LTα1/β2, the predominant heterotrimer, binds and activates only the LT-β receptor, LT-βR. The other heterotrimer, LT-α2/β1 binds and activates TNFR1 and TNFR2 but not LT-βR. TNF-β is expressed in activated T, B and NK cells and in certain transformed cells. Genes for TNF-β, LT-β and TNF-α are tightly linked near each other within the MHC. TNF-β appears to play many of the same biological roles as TNF-α, but the membrane-bound form with LT-β is critical for normal lymphoid organ development. Genetic polymorphisms in TNF-α and TNF-β have been linked to certain pathological conditions, including myasthenia gravis. At the amino acid level, human and mouse TNF-β are 74% homologous and human and mouse LT-β are 80% homologous.