Merck Epidermal Growth Factor, human, animal component free

Epidermal Growth Factor, human, animal component free

EGF, recombinant, expressed in Escherichia coli, >97% (SDS-PAGE)

EGF, Epidermal Growth Factor human

Quality Level

200

생물학적 소스

human

재조합

expressed in E. coli

assay

>97% (SDS-PAGE)

form

lyophilized powder

효능

0.08-0.8 ng/mL EC50

분자량

~6 kDa

포장

pkg of 200 and 500 μg

저장 조건

avoid repeated freeze/thaw cycles

불순물

≤1 EU/μg Endotoxin

색상

white

solubility

water: soluble 0.190-0.210, clear, colorless

UniProt 수납 번호

P01133

저장 온도

−20°C

Gene Information

human ... EGF(1950)

Epidermal Growth Factor (EGF) is a small mitogenic polypeptide (∼6 kDa), which is present in many mammalian species and is distributed throughout a wide number of tissues and body fluids.† Human EGF is identical to β-urogastrone, a polypeptide which was recognized and isolated on the basis of its ability to inhibit gastric acid secretion.† EGF is a member of a growth factor family, which is characterized by the presence of 6 conserved cysteine motifs that form three disulfide bonds. The location of 3 intrachain disulfide bonds in recombinant human EGF is identical to that of mouse EGF.† EGF is homologous to a sequence contained in a 19 kDa protein of vaccinia virus,† which appears to utilize the EGF receptor to gain entry into cell† EGF is mitogenic for a variety of epidermal and epithelial cells, including fibroblasts, glial cells, mammary epithelial cells, vascular and corneal endothelial cells, bovine granulosa, rabbit chondrocytes, HeLa cells, and SV40-3T3 cells.†