Merck hBFGF

hBFGF

FGF-Basic, recombinant, expressed in E. coli, suitable for cell culture

FGF2, bFGF, Fibroblast Growth Factor-Basic human

Quality Level

200

생물학적 소스

human

재조합

expressed in E. coli

assay

≥97% (SDS-PAGE)

form

lyophilized powder

효능

0.05-0.6 ng/mL

분자량

16.0 kDa

포장

pkg of 4X25 μg
pkg of 25 μg

저장 조건

avoid repeated freeze/thaw cycles

technique(s)

cell culture | mammalian: suitable

불순물

≤1.00 EU/μg

색상

white to faint yellow cast

solubility

water: soluble 0.025 mg, clear, colorless to faintly yellow

UniProt 수납 번호

P09038

저장 온도

−20°C

Gene Information

human ... FGF2 (2257)

Fibroblast growth factor-basic (FGF-Basic) is found in basement membranes and sub-endothelial extracellular matrix. FGF-Basic is induced early in the development of the embryo where it is necessary (but not sufficient) for pluripotency and self-renewal of embryonic stem cells (ESC) and lineage defined stem cells; wherein it functions to support proliferation and inhibit differentiation. Self-renewal of hESC depends upon activation of the Activin/Nodal/Smad2,3 branch and suppression of the BMP/GDF/Smad5 branch of the TGFβ-family cell signaling program. FGF-Basic is a competence factor for Nodal support of pluripotency. Activin-A is necessary and sufficient to maintain stemness. It induces the expression of both Nodal and FGF-Basic in human ES cells.