Merck Trypsin from porcine pancreas

Trypsin from porcine pancreas

lyophilized powder, γ-irradiated, BioXtra, suitable for cell culture, 1,000-2,000 BAEE units/mg solid

생물학적 소스

Porcine pancreas

Quality Level

200

무균

γ-irradiated

제품 라인

BioXtra

form

lyophilized powder

specific activity

1,000-2,000 BAEE units/mg solid

분자량

23.8 kDa

농도

25 mg/mL

technique(s)

cell culture | mammalian: suitable

배송 상태

wet ice

저장 온도

2-8°C

For trypsin digestion of peptides, use a ratio of about 1:100 to 1:20 for trypsin:peptide. The typical use for this product is in removing adherent cells from a culture surface. The concentration of trypsin necessary to dislodge cells from their substrate is dependent primarily on the cell type and the age of the culture. Trypsins have also been used for the re-suspension of cells during cell culture, in proteomics research for digestion of proteins and in various in-gel digestionsns†. Additional applications include assessing crystallization by membrane-based techniques and in a study to determine that protein folding rates and yields can be limited by the presence of kinetic traps.