Merck PluriSTEM Dispase-II Solution

PluriSTEM® Dispase-II Solution

Dispase-II has proven to be a rapid and effective, yet gentle, agent for separating many tissues and cells grown in vitro.

Dispase, Dispase 2

Quality Level

100

form

liquid

manufacturer/tradename

PluriSTEM®

농도

1 mg/mL in sterile DMEM/F-12

technique(s)

cell culture | mammalian: suitable
cell culture | stem cell: suitable

배송 상태

dry ice

Proteolytic enzymes such as trypsin, collagenase and pronase are commonly used for dispersing tissues and cells. These enzymes, however, often injure the cells, are unstable during incubation, can be heterogeneous and also a source of mycoplasma contamination. Dispase-II is used for the preparation of cells from a wide variety of different tissues and organs. Dispase-II has proven to be a rapid and effective, yet gentle, agent for separating many tissues and cells grown in vitro. Dispase is especially suitable for tissue disaggregation and subcultivation procedures since it does not damage cell membranes.

PluriSTEM Dispase-II has been validated to work alongside PluriSTEM Human ES/iPS Medium (SCM130) for the culture and passage of human embryonic and induced pluripotent stem cells.

Dispase-II enzyme is produced in Bacillus polymyxa.