Merck EX-CELL 302 Serum-Free Medium for CHO Cells

EX-CELL® 302 Serum-Free Medium for CHO Cells

without L-glutamine, without phenol red, without sodium bicarbonate, dry powder, suitable for cell culture

Quality Level

500

설명

Drug Master File available
for research or for further manufacturing use

무균

sterile-filtered

form

dry powder

저장 조건

protect from light

technique(s)

cell culture | mammalian: suitable

pH

7.1-7.7

저장 온도

2-8°C

EX-CELL® 302 is a serum-free medium, which has been specifically developed for the long-term growth of transformed Chinese Hamster Ovary (CHO) cells in suspension for the expression of antibodies or protein products. EX-CELL 302 is an appropriate medium for use with the DHFR- or Glutamine Synthetase, or GS System™ because it does not contain hypoxanthine, thymidine or L-glutamine. If EX-CELL 302 is to be used with CHO cells transformed using the GS selection system the media needs to be supplemented with GS Supplement 50X for the additional amino acids and nucelosides. For use with the DHFR- selection system we recommend the addition of 4 mM L-Glutamine for optimal growth. Due to current regulatory concerns about the sources of raw materials, EX-CELL 302 was developed using only recombinant human proteins that have molecular weights less than 10 kD. The total protein concentration found in EX-CELL 302 is less than 1 mg/L. Pluronic™ F68 has been added at a final concentration of 0.1% to EX-CELL 302 to protect against shear damage in sparged bioreactor systems.