Merck EX-CELL 302 Serum-Free Medium for CHO Cells

EX-CELL® 302 Serum-Free Medium for CHO Cells

without L-glutamine and phenol red, liquid, sterile-filtered, suitable for cell culture

Quality Level

500

설명

for research or for further manufacturing use

무균

sterile-filtered

form

liquid

저장 조건

protect from light

technique(s)

cell culture | mammalian: suitable

pH

7.1-7.7

성분

L-glutamine: no
HEPES: 7.5 mM
NaHCO3: 1.60 g/L
phenol red: no
glucose: 3.42 g/L

배송 상태

ambient

저장 온도

2-8°C

EX-CELL 302 is a serum-free medium which has been specifically developed for the long-term growth of transformed Chinese Hamster Ovary (CHO) cells in suspension for the expression of antibodies or protein products. EX-CELL 302 is an appropriate medium for use with the DHFR- or Glutamine Synthetase, or the GS System™, because it does not contain hypoxanthine, thymidine or L-glutamine. For use with the DHFR- selection system we recommend the addition of 4 mM L-glutamine for optimal growth. Due to current regulatory concerns about the sources of raw materials, EX-CELL 302 was developed using only recombinant human proteins that have molecular weights less than 10 kDa. The total protein concentration found in EX-CELL 302 is less than 1 mg/L. Pluronic® F68 has been added at a final concentration of 0.1% to EX-CELL 302 to protect against shear damage in sparged bioreactor systems.