Merck Mitochondria Staining Kit

Mitochondria Staining Kit

kit sufficient for 40 tests (of 5 mL cell suspensions), kit sufficient for 200 tests (of 1 mL cell suspensions)

Quality Level

200

사용

kit sufficient for 200 tests (of 1 mL cell suspensions)
kit sufficient for 40 tests (of 5 mL cell suspensions)

포장

pkg of 1 kit

저장 조건

dry at room temperature

technique(s)

flow cytometry: suitable
protein staining: suitable

형광

λex 490 nm; λem 530 nm (green) (JC-1 monomers)
λex 525 nm; λem 590 nm (red) (JC-1 aggregates)

application(s)

cell analysis
detection

detection method

fluorometric

배송 상태

wet ice

저장 온도

2-8°C

In normal cells, the JC-1 dye concentrates in the mitochondrial matrix where it forms red fluorescent aggregates. Any event that dissipates the mitochondrial membrane potential (e.g. apoptosis) prevents the accumulation of the JC-1 dye in the mitochondria and thus, the dye is dispersed throughout the entire cell leading to a shift from red (JC-1-aggregates) to green fluorescence (JC-1 monomers). The fluorescence of the cells stained with this kit may be observed by fluorescence microscopy or measured by fluorimetric and flow cytometry analysis.