Thermo Fisher Scientific Total Oxidant Status (TOS) Colorimetric Assay Kit

Invitrogen™ Total Oxidant Status (TOS) Colorimetric Assay Kit

The Total Oxidant Status (TOS) Colorimetric Assay Kit is a reliable and sensitive assay designed for the quantitative measurement of TOS in cells, tissues, serum, and other liquid sample types.
Oxidant molecules, also known as reactive oxygen species (ROS), are produced during normal metabolic processes and can be further stimulated by internal and external factors. When the balance between oxidation and antioxidant defense shifts toward oxidation, oxidative stress and various diseases may occur.

Measuring concentrations of individual oxidant molecules can be time-consuming, costly, and technically complex. The TOS assay provides a practical solution by quantifying the overall oxidation state of a sample.

Assay Principle

In this assay, oxidizing agents in the sample oxidize ferrous ions (Fe²⁺) to ferric ions (Fe³⁺), which strongly bind to xylenol orange to produce a blue-purple complex with a maximum absorption wavelength around 590 nm.
The color intensity is proportional to the concentration of oxidation substances, allowing indirect calculation of the total oxidation state of the sample.

Key Features

• Simple and fast protocol requiring minimal hands-on time
• Rapid results within a short incubation period
• Broad range of kits for key analytes and metabolic pathways
• Strict quality control for reproducible and reliable outcomes

Specifications