Thermo Fisher Scientific CD33 Monoclonal Antibody (WM53), PerCP
CD33 단백질을 인식하는 WM53 클론의 마우스 모노클로날 항체로, PerCP로 결합되어 유세포분석(Flow Cytometry)에 적합합니다. 인간 및 비인간 영장류 반응성이 있으며, 4°C 암소 보관이 권장됩니다. 연구용으로만 사용 가능합니다.
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Applications
- Flow Cytometry (Flow): 10 µL/1×10⁶ cells
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Non-human primate |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | WM53 |
| Immunogen | Human AML cells |
| Conjugate | PerCP |
| Excitation / Emission Max | 482 / 675 nm |
| Form | Liquid |
| Purification | Size-exclusion chromatography |
| Storage Buffer | PBS with 0.2% BSA |
| Contains | 15 mM sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2534580 |
Additional Formats
Product Specific Information
This antibody reacts with an extracellular epitope of CD33, a 67 kDa type I transmembrane glycoprotein (immunoglobulin superfamily) expressed on myeloid progenitors, monocytes, granulocytes, dendritic cells, and mast cells. It is absent on platelets, lymphocytes, erythrocytes, and hematopoietic stem cells.
Target Information
CD33 is a transmembrane protein of the sialic acid-binding immunoglobulin-like lectin (Siglec) family. It contains immunoreceptor tyrosine-based inhibitory motifs (ITIMs) that recruit protein tyrosine phosphatases SHP-1 and SHP-2, mediating inhibitory signaling and receptor internalization. CD33 is expressed on cells of the myelomonocytic lineage, binds sialic acid residues on cell surface glycans, and serves as a therapeutic target for acute myeloid leukemia. It is present on granulocyte and macrophage precursors in bone marrow but not on pluripotent stem cells, and is a useful marker for peripheral monocytes. CD33 helps distinguish myelogenous leukemia from lymphoid or erythroid leukemias. Diseases associated with CD33 dysfunction include gallbladder lymphoma and extracutaneous mastocytoma.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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