Thermo Fisher Scientific PolarScreen Progesterone Receptor Competitor Assay Kit, Green

Thermo Fisher Scientific PolarScreen™ Progesterone Receptor Competitor Assay Kit, Green

The PolarScreen™ Progesterone Receptor Competitor Assay Kit, Green provides a sensitive and efficient method for high-throughput, fluorescence polarization-based screening of potential progesterone receptor (PR) ligands.
This kit uses insect cell-expressed human PR ligand binding domain tagged with glutathione-S-transferase (GST) and a high-affinity fluorescent progesterone receptor ligand (Fluormone™ PL Green) in a homogeneous, mix-and-read assay format.

This kit produces a green fluorescence readout; a red version is also available. Both kits can determine the IC₅₀ values of competitive compounds. The color selection depends on the spectral properties of the compounds and the available instrumentation.

For additional information or assistance, contact: drugdiscoverytech@lifetech.com

주요 특징

• 모든 시약이 포함된 완전한 포맷 – 시험 화합물만 추가하면 바로 사용 가능
• 고처리량 분석 가능 – 384웰 플레이트 형광 편광 분석 호환
• 대량 스크리닝용 벌크 구성품 제공

Assay 작동 원리

An N-terminal fusion of glutathione transferase to the ligand-binding domain of the human progesterone receptor (PR-LBD(GST)) is added to a fluorescently tagged progesterone ligand (Fluormone PL Green) in the presence of competitor test compounds in microwell plates.
Effective competitors prevent the formation of the PL Green/PR-LBD(GST) complex, resulting in a decrease of the polarization value.
The shift in polarization value in the presence of test compounds is used to determine relative affinity of test compounds for PR-LBD(GST).

Contact us for bulk quantities or for assistance with other nuclear receptor assay needs, from assay development to library screening and beyond.

제품 사양