Thermo Fisher Scientific H3K9me3 Monoclonal Antibody (1-6)
H3K9me3 단백질을 인식하는 Thermo Fisher Scientific의 단클론 항체. Western blot, IHC, ICC/IF 등 다양한 응용에 사용 가능. Human, Mouse, Rat에 반응하며 Protein G로 정제된 액상 형태. 핵 내 히스톤 H3 메틸화 연구에 적합.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:5,000–1:10,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:1000 |
| Immunocytochemistry (ICC/IF) | 1:250 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 1-6 |
| Immunogen | Synthetic peptide within N-terminal human Histone H3 including tri-methyl K9 conjugated to keyhole limpet hemocyanin |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 2 mg/mL |
| Purification | Protein G |
| Storage Buffer | PBS, pH 7.4, with 50% glycerol, 0.2% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2911708 |
Product Specific Information
- Positive Control: NCCIT cell, F9 cell, PC12 cell, A549 cell
- Subcellular Location: Nucleus
Target Information
Histone H3 is one of the DNA-binding proteins found in the chromatin of all eukaryotic cells. H3, along with four core histone proteins, binds to DNA forming the nucleosome structure. Histones play a central role in transcription regulation, DNA repair, DNA replication, and chromosomal stability. Post-translationally, histones are modified in various ways to either directly change chromatin structure or allow binding of specific transcription factors. The N-terminal tail of histone H3 protrudes from the nucleosome core and undergoes several post-translational modifications influencing cellular processes, including methylation, acetylation, and phosphorylation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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