Thermo Fisher Scientific ELK1 Polyclonal Antibody
Thermo Fisher Scientific의 ELK1 Polyclonal Antibody는 인간, 마우스, 랫트 반응성을 가지며 WB, IHC, ICC, ELISA 등 다양한 응용에 적합합니다. KLH 결합 합성 펩타이드로 면역화된 Rabbit IgG 항체로, 고순도 Protein A 정제 및 안정적인 액상 형태로 제공됩니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:300 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:200 |
| Immunohistochemistry (Frozen) (IHC (F)) | 1:100–1:500 |
| Immunocytochemistry (ICC/IF) | 1:50–1:200 |
| ELISA | 1:500–1:1,000 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | KLH conjugated synthetic peptide derived from human ELK1, amino acids 301–428 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | 0.01M TBS, pH 7.4, with 1% BSA, 50% glycerol |
| Contains | 0.02% ProClin 300 |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Target Information
ELK1 is a component of the ternary complex that binds the serum response element (SRE) and mediates gene activity in response to serum and growth factors. It is phosphorylated by MAP kinase pathways at a cluster of S/T motifs at its C-terminus, with phosphorylation at Ser383 being critical for transcriptional activation. ELK1 serves as a nuclear target for the ras-raf-MAPK signaling cascade and is a direct substrate for activated MAP kinase. Alternatively spliced transcript variants encoding the same protein have been identified.
For Research Use Only. Not for use in diagnostic procedures or resale without express authorization.
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