Thermo Fisher Scientific USP15 Polyclonal Antibody

Applications and Tested Dilutions

Product Specifications

Product Specific Information

• Recommended shelf life: 1 year from date of receipt
• For IHC, epitope retrieval with Tris-EDTA pH 9.0 is recommended for FFPE tissue sections
• Based on 100% sequence identity, this antibody is predicted to react with X. tropicalis and Bovine

Target Information

Modification of target proteins by ubiquitin participates in a wide array of biological functions. Proteins destined for degradation or processing via the 26S proteasome are coupled to multiple copies of ubiquitin. Attachment of ubiquitin or ubiquitin-related molecules may also result in changes in subcellular distribution or modification of protein activity.

Deubiquitination is catalyzed by proteases called deubiquitinating enzymes (DUBs), which fall into four distinct families:

1. Ubiquitin C-terminal hydrolases
2. Ubiquitin-specific processing proteases (USPs)
3. OTU-domain ubiquitin-aldehyde-binding proteins
4. Jab1/Pad1/MPN-domain-containing metallo-enzymes

Among these, USPs are the most widespread across evolution. They release ubiquitin from conjugated proteins and share conserved catalytic domains with Cys and His boxes, while their N- and C-terminal extensions contribute to substrate recognition, subcellular localization, and protein-protein interactions.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.