Thermo Fisher Scientific Smooth Muscle Myosin Monoclonal Antibody (SMMS-1), eFluor 660, eBioscience

Applications

Tested Dilution

Publications

Immunohistochemistry (Paraffin) (IHC (P))

20 µg/mL

-

Immunocytochemistry (ICC/IF)

Assay-Dependent

-

Product Specifications

Species Reactivity

Bovine, Dog, Cat, Human, Rat

Host/Isotype

Mouse / IgG1, kappa

Recommended Isotype Control

Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), eFluor™ 660, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

SMMS-1

Conjugate

eFluor™ 660 eFluor™ 660 eFluor™ 660

View additional formats

• Unconjugated
• Alexa Fluor 488
• Request custom conjugation

Excitation/Emission Max

651/668 nm View spectra

Form

Liquid

Concentration

0.2 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2574262

Product Specific Information

Description: This SMMS-1 monoclonal antibody reacts with both isoforms of the human smooth muscle myosin heavy chain (SM1 and SM2) structural proteins, which are components of the contractile apparatus in smooth muscle cells. Smooth muscle myosin heavy chains are expressed by myoepithelial cells and also serve as the terminal differentiation marker for smooth muscle cells. In normal breast tissue, myoepithelial cells compose the outer layer of the lumen lining. In benign and in situ breast lesions, the intact myoepithelial layer stains with SMMS-1 and the loss of this staining indicates invasive cancer. This SMMS-1 antibody has also been reported to cross-react with bovine, canine, feline, and rat smooth muscle myosin heavy chains.

Applications Reported: This SMMS-1 antibody has been reported for use in microscopy, immunohistochemical staining of frozen and formalin-fixed paraffin embedded tissue sections, and immunocytochemistry.

Applications Tested: This SMMS-1 antibody has been tested by immunohistochemistry of formalin-fixed paraffin embedded human tissue using low pH antigen retrieval at less than or equal to 20 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

eFluor™ 660 is a replacement for Alexa Fluor™ 647. eFluor™ 660 emits at 659 nm and is excited with the red laser (633 nm). Please make sure that your instrument is capable of detecting this fluorochome.

Excitation: 633-647 nm; Emission: 668 nm; Laser: Red Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

SM-MHC is a cytoplasmic structural protein, which is a major component of the contractile apparatus in smooth muscle cells. Expression of smooth muscle myosin is developmently regulated, appearing early in smooth muscle development, and is specific for smooth muscle development. Two isoforms of smooth muscle myosin heavy chain have been identified, designated MHC-1 and MHC-2.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.