Thermo Fisher Scientific Phospho-PPAR alpha (Ser12) Polyclonal Antibody
Phospho-PPAR alpha (Ser12) 폴리클로날 항체로 인간, 마우스, 랫트 시료에 반응합니다. WB, IHC, ICC, Flow, ELISA 등 다양한 응용에 적합합니다. KLH 결합 합성 인산화 펩타이드 면역원으로 제작되었으며, 고순도 Protein A 정제 및 안정적 액상 형태로 제공됩니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:200 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:200 |
| Immunohistochemistry (Frozen) (IHC (F)) | 1:100–1:500 |
| Immunocytochemistry (ICC/IF) | 1:50–1:200 |
| Flow Cytometry (Flow) | 1:20–1:100 |
| ELISA | 1:5,000–1:10,000 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | KLH conjugated synthetic phosphopeptide derived from human PPAR alpha around the phosphorylation site of Ser12 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | 0.01M TBS, pH 7.4, with 1% BSA, 50% glycerol |
| Contains | 0.02% ProClin 300 |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Target Information
Peroxisome proliferators are non-genotoxic carcinogens that exert their effects through interaction with nuclear hormone receptors known as peroxisome proliferator-activated receptors (PPARs). These ligand-dependent intracellular proteins stimulate transcription of specific genes by binding to DNA sequences upon activation. PPARs are activated by compounds such as clofibric acid, nafenopin, WY-14,643, and certain fatty acids. They can induce transcription of acyl coenzyme A oxidase and cytochrome P450 (CYP450) A6 through specific response elements. PPARs also heterodimerize with retinoid X receptor (RXR) alpha.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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