Thermo Fisher Scientific PCMT1 Monoclonal Antibody (OTI4A4), TrueMAB

Thermo Fisher Scientific PCMT1 Monoclonal Antibody (OTI4A4), TrueMAB

Applications and Tested Dilution

• Western Blot (WB): 1:500–1:2,000
• Immunohistochemistry (Paraffin) (IHC (P)): 1:500

Product Specifications

Product Specific Information

For reconstitution, add 100 µL distilled water to obtain a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step using Zeba Spin Desalting Columns (7K MWCO, 0.5 mL, Product #89882).

Target Information

Protein carboxyl methyltransferases are classified into three types based on methyl-acceptor substrate specificity in both prokaryotic and eukaryotic cells.
Type II enzyme transfers a methyl group from S-adenosyl-L-methionine to the free carboxyl groups of D-aspartyl and L-isoaspartyl residues, which arise from spontaneous deamidation, isomerization, and racemization of normal L-aspartyl and L-asparaginyl residues.
These methyl-accepting residues represent sites of covalent damage to aging proteins.
PCMT1 (EC 2.1.1.77) functions as a protein repair enzyme that converts abnormal D-aspartyl and L-isoaspartyl residues back to the normal L-aspartyl form.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.