Thermo Fisher Scientific CHRM1 Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:200

-

Immunohistochemistry (IHC)

Assay-dependent

-

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

GST fusion protein with the sequence corresponding to amino acid residues 227-353 of human M1, 3rd intracellular loop if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:CHRM1,uniProtId:P11229-1,ncbiNodeId:9606,antigenRange:227-353,antigenLength:460,antigenImageFileName:AMR-001-200UL_CHRM1_P11229-1_Rabbit.svg,antigenImageFileNamePDP:AMR-001-200UL_CHRM1_P11229-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Concentration

0.95 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.4, with 1% BSA

Contains

0.05% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

Product Specific Information

Immunogen sequence: GSETPGKGGG SSSSSERSQP GAEGSPETPP GRCCRCCRAP RLLQAYSWKE EEEEDEGSME SLTSSEGEEP GSEVVIKMPM VDPEAQAPTK QPPRSSPNTV KRPTKKGRDR AGKGQKPRGK EQLAKRK

Reconstitution: 25 µL, 50 µL or 0.2 mL double distilled water (DDW), depending on the sample size. The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20C. The reconstituted solution can be stored at 4C for up to 1 week. For longer periods, small aliquots should be stored at -20C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Information

The muscarinic cholinergic receptors belong to a larger family of G protein-coupled receptors. The functional diversity of these receptors is defined by the binding of acetylcholine and includes cellular responses such as adenylate cyclase inhibition, phosphoinositide degeneration, and potassium channel mediation. Muscarinic receptors influence many effects of acetylcholine in the central and peripheral nervous system. The muscarinic cholinergic receptor 1 is involved in mediation of vagally-induced bronchoconstriction and in the acid secretion of the gastrointestinal tract. The gene encoding this receptor is localized to 11q13.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.