Thermo Fisher Scientific Rat IgG2a kappa Isotype Control (eBR2a), Brilliant Ultra Violet 496, eBioscience

Applications

Flow Cytometry (Flow)

• Tested Dilution: Assay-Dependent

Control (Ctrl)

• Tested Dilution: Assay-Dependent

Product Specifications

Additional Available Formats

• Unconjugated
• Alexa Fluor 488, 532, 561, 647, 660, 700
• APC, APC-eFluor 780
• Biotin
• Brilliant UV 395, 563, 615, 661, 737, 805
• Brilliant Violet 421, 480, 605, 650, 711, 786
• eFluor 450, 506, 570, 615, 660
• FITC
• Functional Grade
• Pacific Orange
• PE, PE-Cyanine5, PE-Cyanine5.5, PE-Cyanine7, PE-eFluor 610
• PerCP-Cyanine5.5, PerCP-eFluor 710
• Super Bright 436, 600, 645, 702, 780
• Custom conjugation available upon request

Product Specific Information

• Description: Monoclonal rat IgG2a kappa is useful as an isotype control immunoglobulin.
• Applications Reported: Suitable for flow cytometric analysis.
• Applications Tested: Tested on mouse splenocytes and human peripheral blood cells; use at same concentration as experimental antibody.
• Fluorochrome: Brilliant Ultra Violet™ 496 (BUV496) emits at 496 nm; for cytometers with UV (355 nm) laser.
• Staining Buffer Recommendation: When using multiple polymer dye-conjugated antibodies, use Super Bright Complete Staining Buffer (Product # SB-4401-42) or Brilliant Stain Buffer™ (Product # 00-4409-75) to minimize non-specific interactions.
• Light Sensitivity: Protect from light due to photo-induced oxidation.
• Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222-49) or 1-step Fix/Lyse Solution (Product # 00-5333-54) for up to 3 days at 4°C in the dark.
• Excitation/Emission: 350 nm / 496 nm (Ultraviolet Laser).

BRILLIANT ULTRA VIOLET™ is a trademark of Becton, Dickinson and Company, used under license. Powered by Sirigen™.

Target Information

Rat IgG2a is an immunoglobulin subclass produced by the rat immune system.
It activates complement and mediates antibody-dependent cell-mediated cytotoxicity (ADCC).
Molecular weight is approximately 160 kDa, composed of two heavy and two light chains.
Commonly used as control or reference antibody in flow cytometry and immunohistochemistry.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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