Thermo Fisher Scientific 4-Hydroxy-2-hexenal Monoclonal Antibody (5C11.1), APC
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4-Hydroxy-2-hexenal에 특이적인 마우스 단클론 항체로, APC로 결합되어 형광 분석에 적합합니다. Western blot, ELISA, Dot blot에 1:1,000 희석 비율로 사용 가능합니다. 651/660 nm의 형광 특성을 가지며, 단백질 G로 정제된 액상 형태입니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| ELISA | 1:1,000 |
| Dot Blot (DB) | 1:1,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Chemical |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 5C11.1 |
| Immunogen | Synthetic 4-Hydroxy-2-hexenal modified Keyhole Limpet Hemocyanin (KLH) |
| Conjugate | APC |
| Excitation / Emission Max | 651 / 660 nm |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | 95.64 mM phosphate / 2.48 mM MES, pH 7.4, with 0.5 M EDTA |
| Contains | No preservative |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2932311 |
Additional Formats
- Unconjugated (MA5-27576)
- FITC (MA5-45858)
- PE (MA5-45860)
- PerCP (MA5-45859)
- Custom conjugation available upon request
Product Specific Information
- A 1:1,000 dilution of MA5-45857 detects 4-Hydroxy-2-hexenal in 1 µg of 4-Hydroxy-2-hexenal-BSA conjugate by ECL immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.
- Specific for 4-Hydroxy-2-hexenal.
- Slight cross-reactivity with 4-Hydroxynonenal.
- Does not cross-react with Acrolein, Crotonaldehyde, Hexanoyl Lysine, Malondialdehyde, or Methylglyoxal modified proteins.
Target Information
4-Hydroxynonenal (4-HNE, C9H16O2) is an alpha, beta-unsaturated hydroxyalkenal produced by lipid peroxidation in cells. It is the primary alpha, beta-unsaturated hydroxyalkenal formed during this process.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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