Thermo Fisher Scientific 4-Hydroxy-2-hexenal Monoclonal Antibody (12F4), APC
4-Hydroxy-2-hexenal 단백질을 검출하기 위한 APC 결합 단클론 항체. Western blot, ELISA, Dot blot에 사용 가능. 4-Hydroxy-2-hexenal에 특이적이며 4-Hydroxynonenal에 약한 교차 반응. 액상 형태로 4°C 보관.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| ELISA | 1:1,000 |
| Dot Blot (DB) | 1:1,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 12F4 |
| Immunogen | Synthetic 4-Hydroxy-2-hexenal modified Keyhole Limpet Hemocyanin (KLH) |
| Conjugate | APC (Allophycocyanin) |
| Excitation / Emission Max | 651 / 660 nm |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
A 1:1,000 dilution of MA5-45861 was sufficient for detection of 4-Hydroxy-2-hexenal in 1 µg of 4-Hydroxy-2-hexenal-BSA conjugate by ECL immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.
Specific for 4-Hydroxy-2-hexenal. Slight cross-reactivity with 4-Hydroxynonenal.
Does not cross-react with Acrolein, Crotonaldehyde, Hexanoyl Lysine, Malondialdehyde, or Methylglyoxal modified proteins.
Target Information
4-Hydroxynonenal (4-HNE, C9H16O2) is an alpha,beta-unsaturated hydroxyalkenal produced by lipid peroxidation in cells.
4-HNE is the primary alpha,beta-unsaturated hydroxyalkenal formed in this process.
For Research Use Only. Not for use in diagnostic procedures.
Not for resale without express authorization.
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