Thermo Fisher Scientific USP9X Recombinant Rabbit Monoclonal Antibody (JG35-11)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000-1:2,000

-

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:200

-

Immunocytochemistry (ICC/IF)

1:50-1:200

-

Flow Cytometry (Flow)

1:50-1:100

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Rabbit / IgG

Expression System

HEK293 cells

Class

Recombinant Monoclonal

Type

Antibody

Clone

JG35-11

Immunogen

Recombinant protein within Human USP9x aa 1-118

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

TBS, pH 7.4, with 40% glycerol, 0.05% BSA

Contains

0.05% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles, store in dark

Shipping conditions

Wet ice

RRID

AB_2848639

Product Specific Information

Positive Control: SiHa, A549, 293, mouse colon tissue, 293T, F9, human tonsil tissue, mouse fallopian tube tissue.

Target Information

USP9X is an ubiquitin-specific protease involved in the removal and recycling of ubiquitin from proteins degraded in proteasomes. As a deubiquitinating enzyme, USP9x has a critical function in tight junction biogenesis by controlling the levels of the exchange factor for Arf6 (EFA6), a protein shown to facilitate tight junction formation during a narrow temporal window preceding the establishment of cell polarity. USP9X is also located on the X-chromosome but escapes X-inactivation, and defects in USP9X may cause gonadal degeneration in Turner syndrome.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.