Thermo Fisher Scientific BCR Polyclonal Antibody
BCR 단백질을 인식하는 Thermo Fisher Scientific의 Rabbit Polyclonal Antibody로, Western blot, ICC/IF, Flow cytometry, IP 등 다양한 응용에 사용 가능. Human에 반응하며, 항원 친화 크로마토그래피로 정제된 액상 형태. 연구용으로 4°C 보관.
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Applications and Tested Dilution
| Application | Tested Dilution / Amount |
|---|---|
| Western Blot (WB) | 1:2,000–1:10,000 |
| Immunocytochemistry (ICC/IF) | 1:500–1:2,000 |
| Flow Cytometry (Flow) | 0.5 µg / 1×10⁶ cells in 150 µL |
| Immunoprecipitation (IP) | 10 µg / mg lysate |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Region between residue 1221 and 1271 of human breakpoint cluster region (BCR) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | Tris citrate/phosphate, pH 7–8 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Wet ice |
Product Specific Information
- Recommended shelf life: 1 year from date of receipt
- Based on 100% sequence identity, this antibody is predicted to react with Mouse
Target Information
A reciprocal translocation between chromosomes 22 and 9 produces the Philadelphia chromosome, frequently found in patients with chronic myelogenous leukemia. The chromosome 22 breakpoint for this translocation is located within the BCR gene. This translocation produces a fusion protein encoded by sequences from both BCR and ABL (the gene at the chromosome 9 breakpoint).
Although the BCR-ABL fusion protein has been extensively studied, the function of the normal BCR gene product remains unclear. The BCR protein has serine/threonine kinase activity and acts as a GTPase-activating protein for p21rac. Two transcript variants encoding different isoforms have been identified for this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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