Takara EcoRI restriction enzyme

EcoRI restriction site:

G | AATT C
C TTAA | A

Supplied buffer: H

Effect of DNA methylation: enzyme activity is affected by CG methylase

Reaction temperature: 37°C

More Information

• Source: Escherichia coli RY13
• Concentration: 8–20 units/μl [High concentration: 30–60 units/µl]
• Substrate for unit definition: lambda DNA
• Enzyme activity is affected by CG methylase depending on the sequence following the recognition site
• Star activity: Unrelated sites may often be cut in the presence of a high concentration of glycerol, Mn2+, or DMSO, and at low ionic strength. Enzyme activity reduces to 70 to 80% by the addition of spermine (ca. 0.2 mM), but star activity reduces to 30–50%.
• Each lot undergoes stringent quality testing including overdigestion, genome DNA analysis, ligation-recutting and pKF3 cloning

Additional product information

Please see the product`s Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.

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