Takara FbaI (BclI) restriction enzyme
Flavobacterium balustinum 유래 제한효소로, dam 메틸화 DNA에서는 절단되지 않음. 37°C에서 작용하며, 고품질 QC 테스트를 거침. 고농도 글리세롤이나 DMSO 존재 시 star activity 발생 가능. 연구용으로만 사용.
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FbaI (BclI) Restriction Enzyme
Restriction Site
T | GATC A
A CTAG | T- Supplied Buffer: K
- Effect of DNA Methylation: Enzyme activity is affected by dam methylase; therefore, most DNAs from E. coli are not cleavable.
- Reaction Temperature: 37 °C
Product Specifications
| 항목 | 내용 |
|---|---|
| Source | Flavobacterium balustinum |
| Concentration | 4–12 units/µl |
| Substrate for Unit Definition | N6-methyladenine free lambda DNA |
| Star Activity | Unrelated site may often be cut in the presence of high concentration of glycerol or DMSO, and at alkaline pH or low ionic strength |
| Quality Testing | Overdigestion, genome DNA analysis, ligation-recutting, and pKF3 cloning |
Additional Product Information
Please refer to the product’s Certificate of Analysis for details on storage conditions, product components, and technical specifications.
Kit Components Lists and Certificates of Analysis are available under the Documents tab.
Takara Bio USA, Inc.
United States/Canada: +1.800.662.2566
Asia Pacific: +1.650.919.7300
Europe: +33.(0)1.3904.6880
Japan: +81.(0)77.565.6999
FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES.
© 2023 Takara Bio Inc. All Rights Reserved.
All trademarks are the property of Takara Bio Inc. or its affiliates.
Additional product and intellectual property information is available at takarabio.com.
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