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Takara FbaI (BclI) restriction enzyme
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Takara FbaI (BclI) restriction enzyme

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Flavobacterium balustinum 유래 제한효소로, dam 메틸화 DNA에서는 절단되지 않음. 37°C에서 작용하며, 고품질 QC 테스트를 거침. 고농도 글리세롤이나 DMSO 존재 시 star activity 발생 가능. 연구용으로만 사용.

브랜드
Takara
카테고리
Enzymes
판매단위
pk
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Takara 1045A Fba I (Bcl I), 500 Units pk판매 단위 pk ·
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Takara 1045B Fba I (Bcl I), 2,500 Units pk판매 단위 pk ·
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Takara · Takara FbaI (BclI) restriction enzyme

FbaI (BclI) Restriction Enzyme

Restriction Site

T | GATC A  
A CTAG | T
  • Supplied Buffer: K
  • Effect of DNA Methylation: Enzyme activity is affected by dam methylase; therefore, most DNAs from E. coli are not cleavable.
  • Reaction Temperature: 37 °C

Product Specifications

항목 내용
Source Flavobacterium balustinum
Concentration 4–12 units/µl
Substrate for Unit Definition N6-methyladenine free lambda DNA
Star Activity Unrelated site may often be cut in the presence of high concentration of glycerol or DMSO, and at alkaline pH or low ionic strength
Quality Testing Overdigestion, genome DNA analysis, ligation-recutting, and pKF3 cloning

Additional Product Information

Please refer to the product’s Certificate of Analysis for details on storage conditions, product components, and technical specifications.
Kit Components Lists and Certificates of Analysis are available under the Documents tab.


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FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES.
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All trademarks are the property of Takara Bio Inc. or its affiliates.
Additional product and intellectual property information is available at takarabio.com.

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