Takara FbaI (BclI) restriction enzyme

FbaI (BclI) restriction site:

T | GATC A
A CTAG | T

Supplied buffer: K

Effect of DNA methylation: enzyme activity is affected by dam methylase; therefore, most DNAs from E. coli are not clevable.

Reaction temperature: 37°C

More Information

• Source: Flavobacterium balustinum
• Concentration: 4–12 units/µl
• Substrate for unit definition: N6-methyladenine free lambda DNA
• Star activity: unrelated site may often be cut in the presence of high concentration of glycerol or DMSO, and at alkaline pH or low ionic strength
• Each lot undergoes stringent quality testing including overdigestion, genome DNA analysis, ligation-recutting and pKF3 cloning

Additional product information

Please see the product`s Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.

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