Thermo Fisher Scientific PCMT1 Monoclonal Antibody (OTI4A4)
인간 PCMT1 단백질을 인식하는 Mouse IgG2a 단클론 항체로 Western blot 및 IHC(P) 실험에 적합합니다. 고순도 친화 크로마토그래피 정제, 1 mg/mL 농도, 안정적 PBS/BSA/glycerol buffer 구성으로 연구용 단백질 분석에 최적화된 제품입니다.
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Applications
Western Blot (WB)
- Tested Dilution: 1:500–1:2,000
Immunohistochemistry (Paraffin) (IHC (P))
- Tested Dilution: 1:500
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI4A4 |
| Immunogen | Human recombinant protein fragment (aa 60–285 of PCMT1) produced in E. coli |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Affinity Chromatography |
| Storage Buffer | PBS, pH 7.3, with 1% BSA, 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2724960 |
Target Information
Three classes of protein carboxyl methyltransferases, distinguished by their methyl-acceptor substrate specificity, have been found in prokaryotic and eukaryotic cells.
The type II enzyme catalyzes the transfer of a methyl group from S-adenosyl-L-methionine to the free carboxyl groups of D-aspartyl and L-isoaspartyl residues.
These methyl-accepting residues result from spontaneous deamidation, isomerization, and racemization of normal L-aspartyl and L-asparaginyl residues, representing sites of covalent damage to aging proteins.
PCMT1 (EC 2.1.1.77) is a protein repair enzyme that initiates the conversion of abnormal D-aspartyl and L-isoaspartyl residues to the normal L-aspartyl form.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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