Thermo Fisher Scientific Phospho-CREB-1 (Ser133) Polyclonal Antibody
Phospho-CREB-1 (Ser133) 특이적 폴리클로날 항체로, Western blot, IHC, ICC, Flow cytometry, ELISA 등 다양한 분석에 사용 가능. Rabbit IgG 기반으로 Protein A 정제, 고순도 액상 형태. 인간, 마우스, 돼지, 랫트 반응성. 연구용 전용 제품.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:300 |
| Immunohistochemistry (Paraffin) (IHC-P) | 1:500 |
| Immunohistochemistry (Frozen) (IHC-F) | 1:100–1:500 |
| Immunocytochemistry / Immunofluorescence (ICC/IF) | 1:50–1:200 |
| Flow Cytometry (Flow) | 1:100 |
| ELISA | 1:500–1:1,000 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Pig, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | KLH-conjugated synthetic phosphopeptide derived from human CREB-1 around Ser133 [RP(p-S)YR] |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | 0.01M TBS, pH 7.4, with 50% glycerol, 1% BSA |
| Contains | 0.02% ProClin 300 |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Target Information
CREB (Cyclic AMP response element binding protein) is a 43 kDa basic/leucine zipper transcription factor that binds the cyclic AMP response element (CRE) and activates transcription in response to extracellular signals such as neurotransmitters, hormones, membrane depolarization, and growth or neurotrophic factors.
Activation of CREB depends on phosphorylation at serine 133 via p44/42 MAP kinase, p90RSK, p38 MAP kinase, and MSK1.
Only the phosphorylated form functions as a transcription factor by interacting with CREB binding protein (CBP).
CREB is involved in circadian rhythm synchronization, adipocyte differentiation, and learning and memory.
In humans, the CREB gene is located on chromosome 2q.
Dysfunction of CREB is associated with diseases such as Alzheimer’s disease, histiocytoma, and soft tissue melanoma.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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